Dr. Martin Stehling

Flow Cytometry / FACS

Flow Cytometry Unit

Flow Cytometry is a powerful analysis tool in modern biology and life sciences. The applications are numerous and have contributed to many fields including immunology, cell biology and microbiology.

The flow cytometer performs the analysis by passing thousands of cells per second through laser beams and by capturing the light that emerges from each cell as it passes through. The gathered data can be analyzed statistically by flow cytometry software to report cellular characteristics such as size, complexity, phenotype, and health. In addition, defined subpopulations of cells can be purified for further experiments according to their phenotype.

The Flow Cytometry Unit provides comprehensive and technically sophisticated cell analysis and sorting services to all groups within the institute and thereby support and extend their research. The instrumentation consists of a FACSAria™ high speed cell sorter (BD Biosciences) equipped with three lasers (375 nm, 488 nm, 633 nm) and 9 fluorescence detectors. Up to four subpopulations can be sorted at one time. As special add-ons it contains a water recirculator for sample collection cooling and an Automatic Cell Deposition Unit (ACDU) that allows sorting of single cells onto multiwell plates and slides.

Techniques used include high speed cell sorting, multi-color immunostaining, DNA cell cycle analysis, cell cloning, measurement of apoptosis and fluorescence resonance energy transfer (FRET). Sterile sorting allows subsequent culturing of sorted cells.

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