Contact

Dr. Dagmar Zeuschner
Email: d.zeuschner@mpi-muenster.mpg.de

Team

Dr. Dagmar Zeuschner,
Manager of the service unit

Karina Mildner, technician

Electron microscopy / EM

Electron microscopy facility

Electron microscopy visualizes the ultrastructure of cells and tissue at nanometer scale. Cell types and organelles can be identified by their characteristic morphology and are pictured in their most innate location. Localisation and distribution of certain marker molecules are assessed more precisely with immunogold labelling. Results obtained by electron microscopy are not only illustrative but can also be statistically evaluated; as a result Electron microscopy analysis became essential to strengthen the results achieved with other methods.

Service

We offer full service for the researchers in house, which includes sample fixation and further preparation completed by elucidation at the electron microscope. Throughout the route we keep the dialogue with the scientist and assist also with interpretation and picture lay-out until the final presentation/publication image.

We offer TEM-analyses of:

  1. Conventional chemical fixed material embedded in epon-resin, ultra-thin sectioned
  2. Cryo-immuno labelled material according to the Tokuyasu-method
  3. Rotary shadowing
  4. Negative stain

We are experienced in more EM-related techniques like high pressure-freezing, electron tomography and scanning EM.

Equipment (selection)

  • 120 kV TEM
  • Two ultramicrotomes, one equipped for cryo-sectioning
  • High Pressure Freezer
  • Freeze substitution
  • Metal evaporation for C and Pt/C
  • Critical Point Dryer

Publications (selection)

Seebeck F, März M, Meyer AW, Reuter H, Vogg MC, Stehling M, Mildner K, Zeuschner D, Rabert F, Bartscherer K. (2017). Integrins are required for tissue organization and restriction of neurogenesis in regenerating planarians. Development. 2017 Jan 30.

Uhl B, Hirn S, Immler R, Mildner K, Möckl L, Sperandio M, Braeuchle C, Reichel CA, Zeuschner D, Krombach F. (2017). The Endothelial Glycocalyx Controls Interactions of Quantum Dots with the Endothelium and their translocation across the Blood-Tissue Border. ACS Nano. 2017 Jan 30. doi: 10.1021/acsnano.6b06812. [Epub ahead of print]

Costello JL, Castro IG, Hacker C, Schrader TA, Metz J, Zeuschner D, Azadi AS, Godinho LF, Costina V, Findeisen P, Manner A, Islinger M, Schrader M. (2017). ACBD5 and VAPB mediate membrane associations between peroxisomes and the ER. J Cell Biol. 2017 Jan 20. pii: jcb.201607055. doi: 10.1083/jcb.201607055. [Epub ahead of print]

Ramasamy SK, Kusumbe AP, Schiller M, Zeuschner D, Bixel MG, Milia C, Gamrekelashvili J, Limbourg A, Medvinsky A, Santoro MM, Limbourg FP, Adams RH.(2016) Blood flow controls bone vascular function and osteogenesis. Nat Commun. 2016 Dec 6;7:13601. doi: 10.1038/ncomms13601.

Rehberg M, Nekolla K, Sellner S, Praetner M, Mildner K, Zeuschner D, Krombach F.(2016). Intercellular Transport of Nanomaterials is Mediated by Membrane Nanotubes In Vivo. Small. 2016 Apr;12(14):1818. doi: 10.1002/smll.201670068.

Nekolla K, Kick K, Sellner S, Mildner K, Zahler S, Zeuschner D, Krombach F, Rehberg M. (2016). Influence of Surface Modifications on the Spatiotemporal Microdistribution of Quantum Dots In Vivo. Small. 2016 May;12(19):2641-51. doi: 10.1002/smll.201600071. Epub 2016 Mar 29.

Yamamoto H, Ehling M, Kato K, Kanai K, van Lessen M, Frye M, Zeuschner D, Nakayama M, Vestweber D, Adams RH. Integrin β1 controls VE-cadherin localization and blood vessel stability. (2015). Nat Commun. Mar 10;6:6429.

Zhang M, Schulte JS, Heinick A, Piccini I, Rao J, Quaranta R, Zeuschner D, Malan D, Kim KP, Röpke A, Sasse P, Araúzo-Bravo M, Seebohm G, Schöler H, Fabritz L, Kirchhof P, Müller FU, Greber B.
Stem Cells. 2015 May;33(5):1456-69. doi: 10.1002/stem.1964.

Goudarzi M, Mildner K, Babatz F Riedel D, Klämbt C, Zeuschner D, and Raz E. Correlative Light and Electron Microscopy of rare cell populations in zebrafish embryos using laser marks. Zebrafish. December 2015, 12(6): 470-473. doi:10.1089/zeb.2015.1148.

Zeuschner D, Mildner K, Volkery S, Dierkes M, Rehberg M. Quantum Dots in Transmission Electron Microscopy. Imaging and Microscopy, Nov 2015.

Frye M, Dierkes M, Küppers V, Vockel M, Tomm J, Zeuschner D, Roissant J, Zarbock A, Koh GY, Peters K, Nottebaum AF, Vestweber D. (2015) Interfering with VE-PTP stabilizes endothelial junctions in vivo via Tie-2 in the absence of VE-cadherin. J. Exp. Med. 7. pii: jem.20150718.

Vestweber D, Zeuschner D, Rottner K, Schnoor M. Cortactin regulates the activity of small GTPases and ICAM-1 clustering in endothelium: implications for the formation of docking structures. Tissue Barriers 2013, January/February/March

Wang L, Benedito R, Bixel MG, Zeuschner D, Stehling M, Sävendahl L, Haigh JJ, Snippert H, Clevers H, Breier G, Kiefer F, Adams RH. Identification of a clonally expanding haematopoietic compartment in bone marrow. EMBO Journal 2013, 32(2):219-30

Rehberg M, Leite CF, Mildner K, Horstkotte J, Zeuschner D, Krombach F. Surface chemistry of quantum dots determines their behavior in postischemic tissue. ACS Nano 2012, 6(2):1370-9

Jones C, Denecke J, Sträter R, Stölting T, Schunicht Y, Zeuschner D, Klumperman J, Lefeber DJ, Spelten O, Zarbock A, Kelm S, Strenge K, Haslam SM, Lühn K, Stahl D, Gentile L, Schreiter T, Hilgard P, Beck-Sickinger AG, Marquardt T, Wild MK. A Novel Type of Macrothrombocytopenia Associated with a Defect in Alpha2,3-Sialylation. Am J Pathol 2011, 179(4):1969-77

Borta H, Aroso M, Rinn C, Gomez-Lazaro M, Vitorino R, Zeuschner D, Grabenbauer M, Amado F, Schrader M. Analysis of low abundant membrane-associated proteins from rat pancreatic zymogen granules. J Proteome Res 2010, 9(10):4927-39

Rehberg M, Praetner M, Leite CF, Reichel CA, Bihari P, Mildner K, Duhr S, Zeuschner D, Krombach F. Quantum Dots Modulate Leukocyte Adhesion and Transmigration Depending on Their Surface Modification. Nano Lett. 2010, 10(9):3656-64

Bixel MG, Li H, Petri B, Khandoga AG, Khandoga A, Zarbock A, Wolburg-Buchholz K, Wolburg H, Sorokin L, Zeuschner D, Maerz S, Butz S, Krombach F, Vestweber D. CD99 and CD99L2 act at the same site as, but independently of, PECAM-1 during leukocyte diapedesis. Blood 2010, 116(7):1172-84

Fernandéz-Taboada E, Moritz S, Zeuschner D, Stehling M, Schöler HR, Saló E, Gentile L. Smed-SmB, a member of the LSm protein superfamily, is essential for chromatoid body organization and planarian stem cell proliferation. Development. 2010, 137(7):1055-65.

Zeuschner D, Mildner K, Zaehres H, Schöler H.R.(2009) Induced Pluripotent Stem Cells at Nano Scale. Stem Cells Dev 2010, 19(5):615-20

Mari M, Bujny MV, Zeuschner D, Geerts WJ, Griffith J, Petersen CM, Cullen PJ, Klumperman J, Geuze HJ (2008). SNX1 defines an early endosomal recycling exit for sortilin and mannose 6-phosphate receptors. Traffic 2008, 9(3):380-93

Donselaar E, Zeuschner D, Posthuma G, Humbel BM, Slot JW. Immunogold labeling of cryosections from high-pressure frozen cells. Traffic 2007, 8(5):471-85

 

 
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